p19 embryonal carcinoma ec cell line  (ATCC)

Journal: bioRxiv

Article Title: Adenine nucleotide translocase 2 silencing promotes metabolic adaptations and anoikis in P19 embryonal carcinoma stem cells

doi: 10.1101/2024.06.07.597817

Figure Lengend Snippet: Microscopy images of P19 embryonal carcinoma stem cells (P19SCs) and differentiated counterparts (P19dCs) upon 1 µM retinoic acid (RA) treatment for 96h. B) Differentiation (TROMA-I and β-3-Tubulin) and pluripotency (OCT4) markers, and ANT2 protein levels in P19SCs and P19dCs (1 µM, 96h), and C) representative Western blotting images. Ponceau S was used for loading control and normalization purposes. Data as mean ± SEM of optical density (O.D.) expressed as a percentage of P19SCs. Statistical analysis was performed using Student’s t-test. **p < 0.001, ****p < 0.0001 statistically significant to siCtrl.

Article Snippet: P19 embryonal carcinoma cell line was obtained from the American Type Culture Collection (ATCC® CRL-1825TM, RRID:CVCL_2153) and cultured in High glucose Dulbecco’s modified Eagle’s medium (DMEM-D5648, Sigma-Aldrich) supplemented with 10% FBS, 1.8 g/l sodium bicarbonate, 110 mg/l sodium pyruvate, and 1% antibiotic/antimycotic solution, at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Microscopy, Western Blot, Control

Journal:

Article Title: Lsh, an epigenetic guardian of repetitive elements

doi: 10.1093/nar/gkh821

Figure Lengend Snippet: Lsh targets to repetitive sequences. (A) Western analysis of Lsh expression in the embryonal carcinoma cell-line P19 and embryonal fibroblasts (MEFs) by using a specific antibody raised against the C-terminal portion of Lsh. (B) ChIPs were performed on P19 cells using the C-terminal antibody against Lsh or an IgG control. The precipitated material was examined by western analysis using the C-terminal anti-Lsh antibody for detection. (C) ChIPs were performed from P19 chromatin extracts using the C-terminal antibody against Lsh or an IgG control. Precipitated DNA or chromatin input was analyzed for the presence of the indicated repeat sequences. DNA was diluted (1:3) for the detection of linear PCR amplification.

Article Snippet: Embryonal carcinoma cell line P19 was purchased from ATCC and cultured according to the manufacturer's protocol.

Techniques: Western Blot, Expressing, Amplification

Journal: Pharmaceutics

Article Title: Screening for Best Neuronal-Glial Differentiation Protocols of Neuralizing Agents Using a Multi-Sized Microfluidic Embryoid Body Array

doi: 10.3390/pharmaceutics14020339

Figure Lengend Snippet: ( A ) Schematic workflow of neuronal and astrocytic differentiation protocols using retinoic acid (RA) or EC23 as inducers. Diameters of embryoid bodies after one-, two-, three- and four-day treatment with concentrations of non-treated (NT), 0.5 µM, 1.0 µM, and 10 µM of ( B ) RA and ( E ) EC23. Bright-field micrographs of non-treated, ( C ) 10 µM RA-treated, and ( F ) 10 µM EC23-treated P19 embryoid bodies in a microtiter plate after four-day treatment. Scale bar, 100 µm. Viability of embryoid bodies after one-, two-, three- and four days post-treatment with increasing concentrations of ( D ) RA and ( G ) EC23. Statistical significance was tested by using one-way ANOVA (* p < 0.05 and *** p < 0.001), n = 3 ± SD. Bars without * do not represent statistical significance.

Article Snippet: The murine embryonal carcinoma cells line P19 (ATCC, Manasass, VA, USA, CRL-1825) was maintained in Minimum Essential Medium Alpha Modification (α-MEM; Sigma-Aldrich, Vienna, Austria) supplemented with 7.5% newborn calf serum (Sigma-Aldrich, Vienna, Austria), 2.5% fetal bovine serum (Sigma-Aldrich, Vienna, Austria), and 1% antibiotic/antimycotic solution (Sigma-Aldrich, Austria) under standard cell culture conditions at 37 °C in a 5% CO 2 humidified atmosphere.

Techniques: